Axial resolution analysis in compressive digital holographic microscopy

Digital in-line holographic microscopy.

We first briefly review the state of the art of digital in-line holographic microscopy (DIHM) with numerical reconstruction and then discuss some technical issues, such as lateral and depth resolution, depth of field, twin image, four-dimensional tracking, and reconstruction algorithm. We then present a host of examples from microfluidics and biology of tracking the motion of spheres, algae, an...

Digital In-line Holographic Microscopy

Three-dimensional imaging with micron resolution and simultaneous tracking of many (hundreds) objects (bubbles, beads, algae, bacteria etc.) is now possiblein situ not only with a benchtop microscope, but also remotely in such environments as the deep ocean with a submersible version of the instrument. The instrument is very simple in its hardware requirements and an efficient software package ...

Speckle-field digital holographic microscopy.

The use of coherent light in conventional holographic phase microscopy (HPM) poses three major drawbacks: poor spatial resolution, weak depth sectioning, and fixed pattern noise due to unwanted diffraction. Here, we report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging. A speckle beam of a complex ...

Alternative Reconstruction Method and Object Analysis in Digital Holographic Microscopy

Co-design of an in-line holographic microscope with enhanced axial resolution: selective filtering digital holography.

Common-path digital in-line holography is considered as a valuable 3D diagnostic technique for a wide range of applications. This configuration is cost effective and relatively immune to variation in the experimental environment. Nevertheless, due to its common-path geometry, the signal-to-noise ratio of the acquired hologram is weak as most of the detector (i.e., CCD/CMOS sensor) dynamics are ...